cas9 viral particles (Addgene inc)
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cas9 viral particles
Cas9 Viral Particles, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/CAG-Cas9+(Plasmid+%2389995)/pm37828578-118-43-103
Average 96 stars, based on 237 article reviews
Cas9 Viral Particles, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+viral+particles/CAG-Cas9+(Plasmid+%2389995)/pm37828578-118-43-103
Average 96 stars, based on 237 article reviews
cas9 viral particles - by Bioz Stars,
2026-09
96/100 stars
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Transfection:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Control:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Western Blot:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Article Title: Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer Article Snippet: DiFi and LIM1215 cells were transiently transfected with pEZY constructs using Lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol and selected with 0.5 μg/mL Neomycin. siRNA mediated knockdown of NF1 in DiFi and LIM1215 cells was performed using Dharmacon siGenome pool and Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s recommended protocol. .. LIM1215 cells were transduced with shRNA:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Knockdown:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Retroviral:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Transduction:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Article Title: Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer Article Snippet: DiFi and LIM1215 cells were transiently transfected with pEZY constructs using Lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol and selected with 0.5 μg/mL Neomycin. siRNA mediated knockdown of NF1 in DiFi and LIM1215 cells was performed using Dharmacon siGenome pool and Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s recommended protocol. .. LIM1215 cells were transduced with Sequencing:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Expressing:Article Title: Cardiac glycoside ouabain efficiently targets leukemic stem cell apoptotic machinery independent of cell differentiation status. Article Snippet: Lentiviral production was performed using HEK293T packaging cells (American Type Culture Collection, ATCC; Manassas, VA) in conjunction with pCMV.dR8.2 dvpr lentiviral packaging and pCMV-VSV-G envelope plasmids (Addgene #8454 and 8455) [31]. .. Cells were first transfected with viral particles containing sgRNA with fluorescent GFP reporter in the presence of hexadimethrine bromide (HBr; 8 μg/mL) for 48 h and sorted for GFP-positive cells by FACSAria, hereafter designated as iMcl-1 cells. iMcl-1 cells were then transfected with Article Title: Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer Article Snippet: DiFi and LIM1215 cells were transiently transfected with pEZY constructs using Lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol and selected with 0.5 μg/mL Neomycin. siRNA mediated knockdown of NF1 in DiFi and LIM1215 cells was performed using Dharmacon siGenome pool and Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s recommended protocol. .. LIM1215 cells were transduced with Derivative Assay:Article Title: Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer Article Snippet: DiFi and LIM1215 cells were transiently transfected with pEZY constructs using Lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol and selected with 0.5 μg/mL Neomycin. siRNA mediated knockdown of NF1 in DiFi and LIM1215 cells was performed using Dharmacon siGenome pool and Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s recommended protocol. .. LIM1215 cells were transduced with |